IGEM:University of Chicago/2009/Notebook/PNP Biosensor/2009/08/11

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August 11th, 2009

Insert GFP Superfolder PCR (Add on PacI) Gel Image

Nora ran a gel to confirm if PCR worked for both my genomic tests and for my GFP PCR from colonies. Genomic tests yielded an empty gel, so either no integration, misintegration, or technical error. Perform PCR to check

Restriction Digest of GFP for Ligation into Longtine Plasmid

20 ul GFP

          GFP DNA = 10 uL
          Buffer 4 = 2 uL
          BSA (10X) = 2 uL
          ddH2O = 5 uL
          Enzyme: PacI = 0.5 uL
          "" : AscI = 0.5 uL

Control

          GFP-Kan Vector = 1 uL
          Buffer 4 = 2 uL
          BSA 10X = 2 uL
          ddH2O = 14 uL
          PacI = 0.5 uL
          Asc I = 0.5 uL

Digest at 37 degrees celsius Started at 12:35 p.m.


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