IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/07/09
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- Helped Damon/Annie - Ordered Primers for PNP Sensors - plates from 7/7 are empty - will try incubation O/N after heat shock (Chichi's advice) - Salmon sperm. DNA looked defect yesterday. Damon makes new batch. Maybe Kan casette is defect. Try PCR (beads) for His-tag/marker. Each tube (50 ul) - 2 beads - 0.65 ul template (pFA6a-GFP-His from Satoe) - 5 ul of each primer - 37.35 ul dd H2O - Program "iGem 6". Will run on gel. - also will try transformations again w/Kan marker plate tmrw. New Kan plates - Need to order Forward primers for double checking biosensor once yeast grows. Check if correct DNA was incorporated. End of Day
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