IGEM:University of Debrecen:Formula and making SOB

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Scientific background<o:p></o:p>

Super Optimal Broth (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli. It was developed by Douglas Hanahan in 1983 and is an adjusted version of the commonly used lysogeny broth. Growth of E. coli in SOB or SOC medium results in higher transformation efficiencies of plasmids.<o:p></o:p>

Super Optimal broth with Catabolite repression (SOC) is SOB with added glucose.<o:p></o:p>

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Materials<o:p></o:p>

To make 1L SOB media:<o:p></o:p>

  • 20 g Bacto-tryptone<o:p></o:p>
  • 0.5 g NaCl<o:p></o:p>
  • 5 g Yeast extract<o:p></o:p>
  • 900 ml distilled H2O<o:p></o:p>

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Procedure<o:p></o:p>

  1. Shake until the solution dissolves.<o:p></o:p>
  2. Add 10 ml 250mM KCl.<o:p></o:p>
  3. Adjust pH to 7.0 with 10N NaOH.<o:p></o:p>
  4. Bring to a final volume of 1L with H2O. Pour into 100 ml bottles and autoclave.<o:p></o:p>
  5. To each bottle, add 500 ul 2M MgCl2.<o:p></o:p>
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