IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/01

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Week 3 Day 2 TSL - Selection of 2 white colonies from 30.06.14 Dig-Lig transformed plates for a colony PCR reaction. The same 2 colonies were grown in 5 ml of SOC broth O/N at 37°C. - Poured a 1% agarose gel using TBE buffer with a 1:20,000 dilution of ethidium bromide. - Gel run with Dig-Lig PCR products.