IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/23
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Week 2 Day 1
- Completed TSL safety induction
- Collected synthesised samples from DNA 2.0 and transformed DNA into E.coli using electrocompetent cells and electroporation. Added 500 μl of Soc broth and incubated for 40 minutes at 37 °C. Spreadplated onto agar plates containing Kanamycin and incubated O/N at 37 °C.