IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/28

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Week 7 Day 1

- Digest of PRO, CDS and TER with Bsa1

- Set up level one dig-ligs 1, 5, 9, 15, 16, 17, 18, 19 and PCR.

- Agrobacterium transformation. 2 μl DNA into 50 μl of cells followed by electroporation. Incubation in LB for 2 hours. Spreadplated onto LB containing: 1 μl/ml of Ampicillin (100 mg/mL), 1 μl/ml of Rif (25 mg/mL) and 1 μl/ml of Gentamycin (10 mg/mL). Incubated overnight at 30 °C.



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