IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/24

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Week 6 Day 4

- Mini-prep of O/N cultures grown on 23/07/14 of DNA synthesised by DNA 2.0.

Tubes 1 to 6:

1. AtPR1 Conc: 182.2 ng/µl 260/280: 1.94

2. ArvBS3 Conc: 342.7 ng/µl 260/280: 1.93

3. NbBi1-RNA Conc: 152.2 ng/µl 260/280: 1.95

4. NbProB-RN Conc: 181.6 ng/µl 260/280: 1.90

5. AvrXa27 T Conc: 263.2 ng/µl 260/280: 1.90

6. OsXa27 Pro Conc: 286.5 ng/µl 260/280: 1.90

- DNA quantified using Nanodrop and blanked with EB buffer.

- Further mini prep of O/N cultures grown on 22/07/14 and 23/07/14 from the BioBricks requested from the 2010 Nevada team's (http://2010.igem.org/Team:Nevada) project 5 mL was analysed from an inoculation of 10 mL LB + CAM.

Biobricks obtained are: BBa_K414001, BBa_K414003, BBa_K414004 and BBa_K414008

They were run on a 1% agarose gel with TAE buffer.



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