IGEM:University of Groningen/2011/Notebook/Count coli/2011/08/11

Thu 11 August 2011

To Do List

• Plasmid prep and send for sequencing
  • 12 variants autoinducing loop
  • RFP internal control
  • construct 21.12
• Pick colonies of back up transformation ---Growth ON culture

Implementation

Result 1st batch transformation
all of 22.xx and 21.12 plasmids have no insert

RBS-RFP PCR colonies screening --> pick 8 colonies --> ALL POSITIVE! *\(^___^)/* Hoera!! -> isolate the plasmids and send for sequencing!

Highlight

• Weekly team meeting at 1 pm
• Modelling lecturer from Prof. Matthias Heinemaann