IGEM:University of Groningen/2011/Notebook/Count coli/2011/08/11

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Count coli Project description
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Thu 11 August 2011

To Do List

  • Plasmid prep and send for sequencing
    • 12 variants autoinducing loop
    • RFP internal control
    • construct 21.12
  • Pick colonies of back up transformation ---Growth ON culture


Result 1st batch transformation
all of 22.xx and 21.12 plasmids have no insert

RBS-RFP PCR colonies screening --> pick 8 colonies --> ALL POSITIVE! *\(^___^)/* Hoera!! -> isolate the plasmids and send for sequencing!


  • Weekly team meeting at 1 pm
  • Modelling lecturer from Prof. Matthias Heinemaann

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