IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/27

UIUC - Arsenic Collection System!

Main project page Previous entry      Next entry

8/27/10 10:00 AM gel Lane Sample Amount 1 1 Kb Ladder 1 μL 2 ArsB1 5 μL sample + 1 μL dye 3 ArsB2 5 μL sample + 1 μL dye 4 ArsB3 5 μL sample + 1 μL dye 5 ArsB4 5 μL sample + 1 μL dye 6 Negative control 5 μL sample + 1 μL dye 11:30 AM PCR Ingerdient Amount Mastermix (6 reactions) 10X Buffer 2 μL 12 μL 10 mM dNTPs .4 μL 2.4 μL Template .9 μL (18 ng) added individually to each reaction F Primer .4 μL 2.4 μL R Primer .4 μL 2.4 μL Polymerase .3 μL 1.8 μL H2O 14.6 μL 87.6 μL DMSO 1 μL 6 μL Samples: ArsB 1 ArsB 2 ArsB 3 ArsB 4 negative control (no template) Primers: ArsB F 2.0, ArsB R 2.0 at 10 micromolar Program: 95°C 3 min 95°C 30 sec 53°C 30 sec 72°C 2 min go to step 2 32X 72°C 5 min hold at 4°C 4:14 PM restreaked ArsRSDM2 made 5mL overnight of ArsRSDM2-B0015 colony.