IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/11

UIUC - Arsenic Collection System!

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9/11/10 10:15 AM Digestion B0034ArsRSDM2B0034pSB1C3 DNA 1.8 μL H2O 39.7 μL Buffer 4 5 μL BSA .5 μL XbaI 1 μL PstI 2 μL J23100 DNA 2.6 μL H2O 39.9 μL Buffer 4 5 μL BSA .5 μL EcoRI 1 μL SpeI 1 μL pSB1T3 DNA 1 μL H2O 40.5 μL Buffer 4 5 μL BSA .5 μL EcoRI 1 μL PstI 2 μL incubate at 37°C for 15 min, inactivate at 80°C for 20 min 11:10 AM Ligation B0034ArsRSDM2B0015 2 μL J23100 2 μL pSB1T3 2 μL Ligase Buffer 2 μL Ligase 1 μL H2O 11 μL Incubate at room temp for 15 min, inactivate at 80°C for 20 min