IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Lock and Key Decoder/2010/08/11

From OpenWetWare

Jump to: navigation, search
iGEM Project name 1 Main project page
Previous entry      Next entry

8/11

  • Pulled pLac promoter (R0010) from the 37°C room. - 11:00 a.m.

Transformation

  • Transformed sub-system 2a: - 3:45 p.m.
    • 50 μL competent cells
    • 4 μL 2a plasmids
    • 10 minutes at 0°C
    • 45 seconds at 42°C
    • 2 minutes at 0°C
    • Added 1000 μL SOC broth
    • 1 hour on shaker in 37°C room
    • plated onto Tetracycline and LB growth media and left in 37°C room overnight

Inoculation

  • inoculated pLac promoter and GFP - 5:00 p.m.
    • left in 37°C room overnight
  • Matthew Entler 17:02, 11 August 2010 (EDT):



Personal tools