IGEM:VGEM/2007/Notebook/2007-6-22

From OpenWetWare

Jump to: navigation, search

Friday June 22, 2007

AM

  • George's paper presentation
  • Observed multiple pink colonies of RFP-transformed (Z-competent) cells on agar plate.
  • Observed pinkish broth that was left in shaker incubator overnight @ ~36.5 +/- 0.5 degrees C. Unable to excite red fluorescence with standard long wave UV light (which is optimal for GFP excitation).
  • Cryogenic freezing of transformed cells from broth (DH5alpha E. coli)
    1. 1 mL of glycerol
    2. 1 mL of cell-containing broth
    3. Filled cryogenic tube (2 mL total)
    4. Vortexed at level 6 for ~ 6-10 secs
  • Making broth from transformed Z-competent cells
    1. 5 mL of LB broth
    2. Added scraping of cells to broth (use sterilized wire-loop)
    3. Vortexed at level 6 for ~6-10 secs
    4. Incubated in shaker @ ~36.5 +/- 0.5 degrees C.

PM

  • Detailed research in photosignalling systems and other possible photoreceptors/ photosensors.
  • (Next week's paper presentations will focus on photobiology of microorganisms)
Personal tools