IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/09/30

Week of 9/30/12

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Week of 9/30/12 Gel of the 12 PCR products showed no sign of the vector. Repeated gel with P+RBS+SynCG plasmid #2 and plasmid #5, along with the 5 plasmid of P+RBS+SynCG from last week. Performed pregnancy test experiments with SynCG biobricks in DH5α with two trials: one with only the #2 culture (the culture which the biobrick was extracted from) and one from the combined culture. These cultures were separated for sonication. Based off of previous results, no DTT will be added, only 8M Urea and no denaturing solution.