IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/20

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July 20th, 2010

  • Prepared gel electrophoresis dyes
  • Digested CFP construct for gel electrophoresis
  • Ran three gels with CFP construct
  • Excised band thought to be CFP construct
  • Repeated CuSO4 treatments on E. coli
  • Aliquoted 1 ml of the CuSO4 treated cultures (above) to microcentrifuge tubes, centrifuged, and observed pellets for fluorescence with UV lamp
  • Read optical density of E. coli growing in M9 media
  • Prepared an overnight culture of GFP in LB Carb broth
  • Autoclaved biohazard waste and pipette tips

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