IGEM:Wisconsin-Madison/2009/Notebook/Spring Training 2012/2012/02/29

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Sequencing the pfu plasmid for iGEM cloning training

  • cleanSEQ of Andrew's pfu plasmid using Matt's pET Up primer and Andrew's T7 terminator reverse primer

10 ul Big Dye reaction

  • 1.5 ul Big dye buffer
  • 1 ul Big dye
  • 1 ul primer
  • 1 ul DNA (171 ng/ul pET1a pfu)
  • 5.5 ul water

"seq50" on iGEM block A

  • 95°C for 3:00
    • 51 repeats of:
  • 95°C for 0:30
  • 50°C for 0:10
  • 60°C for 4:00

Cleaned up and eluted in 20 ul nuclease free H2O.


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