IGEM:Yale/2010/Notebook/2010/06/19

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Transformation failure :(

  • None of the incubated BL21 tranformant plates showed growth, so redid transformation of 6/18, this time using competent LE392 cells, according to standard protocol. After plating 100 uL of the resulting solution, saved the remainder at 4 C in case of another failure.
  • Culture of Addgene cells containing pSB74 showed growth, so set aside at 4 C, planning to inoculate liquid cultures on the 20th.


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