From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Transformation failure :(

  • None of the incubated BL21 tranformant plates showed growth, so redid transformation of 6/18, this time using competent LE392 cells, according to standard protocol. After plating 100 uL of the resulting solution, saved the remainder at 4 C in case of another failure.
  • Culture of Addgene cells containing pSB74 showed growth, so set aside at 4 C, planning to inoculate liquid cultures on the 20th.

Personal tools