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First PCR and other excitement

  • Inspected plated cultures of DH5alpha, BL21, and LE392 transformed with pSB74 onto Amp plates. LE392 and DH5alpha showed successful transformation, but no transformant colonies were seen of BL21. Given failure of previous BL21 transformation noted on the 19th , concluded that BL21 cells from Modis lab were not actually competent.
  • Checked on fresh LB plates incubated over weekend as a sterility check--no growth!

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