IGEM:Yale/2010/Notebook/2010/07/04

Project name

Transformed Colonies from Ligation Attempt #2

phsABC/B0015 system
Reaction #1 (control)	2 colonies
Reaction #2 (2:1 insert:vector)	4 colonies, numbered 1B-4B
Reaction #3 (2:1 insert:vector)	5 colonies, numbered 5B-9B
Reaction #4 (large control)	0 colonies
J23114/P0312 system
Reaction #5 (control)	numerous* colonies
Reaction #6 (2:1 insert:vector)	numerous colonies, somewhat more than #5
Reaction #7 (3:1 insert:vector)	numerous colonies,about double #5
Reaction #8 (5:1 insert:vector)	numerous colonies, about double #5

numerous=more than readily countable

Inoculated 5 mL liquid Amp/LB cultures with colonies 1B-9B and let grow overnight at 37 on shaker.
Set aside plates 6-8 in the fridge to work with later.

This day's activities are also recorded on page 46 in the hard copy of the lab notebook.