IGEM:Yale/2010/sandbox

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Digestion Reaction Components
phsABC DigestionB0015 DigestionpSB1C3 Digestion
5 μL EcoRI Buffer 10x 5 μL NEB Buffer 3 10x 5 μL EcoRI Buffer 10x
0.5 μL BSA 100x
21.1 μL phsABC soln (1 μg DNA) 3.8 μL B0015 soln (1 μg DNA) 15 μL phsABC soln (375 ng DNA)
1.8 μL EcoRI & 1.8 μL SpeI 1.8 μL XbaI & 1.8 μL PstI 1.2 μL EcoRI, 1.2 μL PstI, & 1.2 μL DpnI
20.8 μL H2O 37.1 μL H2O 15.9 μL H2O


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