IGEM:metu/2009/Notebook/wound dressing/2009/07/18

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Image: Wound dressing logo.jpg

18.07.2009

1. Measurement of OD with spectrophotometer (600 nm)

Dilution factor : 1/5

OD results; Backbone 1 = 0.098 Backbone 2 = 0.038 Backbone 3 = 0.034

They were very low, so we will make plasmid isolation again.

2. Preparation of Electrophoresis Gel

0.5 gr Agarose + 50 ml  1×TAE buffer + 4 µl EtBr 
We added EtBr after cool down.
TAE electrophoresis buffer = 50×            
We used 1x TAE.  (20ml TAE + 980 ml dH2O)

3. Electrophorese was done.

Ladder = 5 µl.
2 µl plasmid + 3 µl dH2O + 1 µl loading dye
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