IGEM:metu/2009/Notebook/wound dressing/2009/08/03

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Image: Wound dressing logo.jpg

03.08.2009

1. 15:10 --- Transformation

EGF /  BBa_Q04400_ By Caitlin Conbay_ 2009 plate 1-16 P
EGF /  BBa_B0015 By Reshma Shetty- 2009 plate 1- 23L
KGF /  BBa_F1610_ 2009 plate 2-24 G
KGF /  BBa_R0011- 2009 plate 1- 6 G

2. 17:00 --- measurement of nanodrop

3. 18.00 --- In order to make plasmid isolation, we put 10 ml LB+Amp + Single transformed backbone colony for 16 hours incubation.

4. 18:00 – DIGESTION

Double digestion
Backbone     prefix: EcorI & suffix : PstI = Buffer O
Lysis        XbaI and PstI = 1x tango, 2 fold PstI
(procedure : fermentas)
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