IGEM:metu/2009/Notebook/wound dressing/2009/09/07

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Image: Wound dressing logo.jpg

07.09.2009

1. Isolations of 4.07,2.09,1.09,2.07,5,1.07,8.07,6,10M Lysis,7,9,BB were made.

2. Digestions were made. The following amounts are added to microtubes;

   1.07,1.09 ,            Buffer Tango         2 µl         
                          Spe1                 2 µl         
                          DNA                 16 µl  =20 µl
                           
   2.07,2.09 ,            Buffer O             2 µl         
                          Pst1                 2 µl         
                          DNA                 16 µl  =20 µl
                 
   1.09 control,          Buffer Tango         1 µl         
                          Spe1                 0 µl         
                          DNA                  9 µl  =10 µl
  
   BB,2.09 control,       Buffer O             1 µl         
                          Pst1                 0 µl         
                          DNA                  9 µl  =10 µl



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