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1. Dissolve 10 mg genipin (cross-linker for collagen) in 1 ml 70 % ethanol (the concentration of genipin solution is 1 %)
2. Add 650 µl genipin solution to the collagen solution (final concentration of genipin solution in the collagen solution is 0,1 %)
3. Place 1 ml prepared final solution to well
4. Wait the prepared solution in the wells for 48 hours at room temperature.