IMPERIAL/2007/Projects/Cell by date/List of Required Parts
- T7 Promoter
Short description: To be used with T7 polymerase BBa_J34811
- pBad Promoter
pBad is an E. coli promoter that is tightly controlled by:
Repressor: AraC apparently acts as the repressor
One study concluded that arabinose can change the conformation of araC and prevent it from successfully binding to and repressing pBad.
- RBS (Elowitz)
Short description: This is the most efficient RBS in the registry, and sets the 1.0 efficiency standard. It is based on the Elowitz repressilator.
- Double terminator (B0010-B0012)
Short description: Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable. forward_efficiency: 0.984 reverse_efficiency: 0.295
- Plasmid Vactor pBAC874t
- T7 RNApol
Short description: To be used with specal tRNA loading glutamate instead of stop codon (Part BBa_J34812).
- tRNA loading Glutamate on stop codon TTT