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1% Agarose gel containing self-assembly reactions of dome and plate, both purified and unpurified as well as samples where the two origamies are attempted connected. All self-assembly reactions were performed in 12.5mM MgCl2 over a 17 hour non-linear ramp. Lane 1: 1kb Ladder, Lane 2: M13 control, Lane 3: Unpurified 21nM plate, Lane 4: 21nM plate purified with spin filter, Lane 5: Unpurified 1nM dome, Lane 6: 1nM dome up-concentrated, but not purified, using spin filter, Lane 7: Up-concentrated and freeze nā€™ squeeze purified 1nM dome, Lane 8: Connection attempt at room temperature, Lane 9: Connection attempt at 2.5 hour linear ramp.

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current04:41, 20 October 2013496Ɨ475 (162 KB)Veronica Liv Andersen (Talk | contribs) (1% Agarose gel containing self-assembly reactions of dome and plate, both purified and unpurified as well as samples where the two origamies are attempted connected. All self-assembly reactions were performed in 12.5mM MgCl2 over a 17 hour non-linear ramp)

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