Aseptically put 2 x 5 ml of LB media into a 2 x 30 ml sterilin universal (pre-sterilised). Add 5 l of ampicillin antibiotic (50 mg/ml). (Question 1: What is the final concentration of ampicillin in the overnight culture ?)
Inoculate each 5 ml culture from a different single E. coli colony from the plate by picking and inoculating with a sterile loop.
Label each tube with your name and a number to differentiate each culture.
Place the cultures in the 28°C incubator for overnight growth.