Imperial College/Courses/Spring2008/Synthetic Biology/Part Measurement and Characterisation/Resources

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Spring 2008 - Introduction to Synthetic Biology

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Wet Lab: Part Measurements and Characterisation




Calibrated Measurements and Calibration Curves:

Promoter Characterisation using a standardised measurement kit

  • Each group is given X promoters to sort out by strength.
  • Detailed protocol to be given.
  • Collect data, and document their work in their OWW lab notebook.
  • BioBrick candidates:
    • <bbpart>I20259</bbpart>: Constitutive_Promoter_1 (from combinatorial library) --GFPmut3--XXX
    • <bbpart>I20260</bbpart>: Constitutive_Promoter_2 (from combinatorial library) --GFPmut3--XXX
    • <bbpart>I20261</bbpart>: Constitutive_Promoter_3 (from combinatorial library) --GFPmut3--XXX
    • <bbpart>I7101</bbpart>: pTetR--GFPmut3--XXX
    • <bbpart>I7107</bbpart>: pLacI--GFPmut3--XXX
    • <bbpart>I7109</bbpart> pCI--GFPmut3--XXX
  • Useful protocols:


Inducible Promoter Characterisation

  • Using the previous protocols, students are expected to define their own standard protocol.
  • Collect data and document their work in their OWW lab notebook.
  • Model expression system, Analyze Data.
  • BioBrick candidates:
    • <bbpart>I0500</bbpart>: pBAD promoter (only)
    • <bbpart>I13520</bbpart>: pBAD--mRFP--XXX
    • <bbpart>I6058</bbpart>: pBAD--EYFP--XXX
    • <bbpart>I13540</bbpart>: pBAD--GFPmut3--XXX
  • Useful Protocols:


Inverter Characterisation

  • From the previous protocols students have to define their own standard protocol.
  • Collect data and document their work in their OWW lab notebook.
  • Model expression system, and Analyze Data.
  • BioBrick candidates:
    • <bbpart>J45996</bbpart>: Exponential-Phase-Sensitive GFP Generator
    • <bbpart>J45995</bbpart>: Stationary-Phase-Sensitive GFP Generator
    • <bbpart>J5517</bbpart>: pBAD--LacIts--XXX--pLacI--GFP--XXX
    • <bbpart>E0611</bbpart>: pBAD--tetR-Inverter--EYFP--XXX
    • <bbpart>E0610</bbpart>: pBAD--LacI-Inverter--EYFP--XXX
    • <bbpart>E0612</bbpart>: pBAD--cI-Inverter--EYFP--XXX
    • <bbpart>pSB1A10</bbpart>: Screening plasmid (GFP-inputs/RFP-outputs)+ TetR-Inverter
  • Useful Protocols:


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