Integrases/Excisionases pairs to test
We will start to test 3 pairs of the serine integrase family. Integrases from this family are interesting because they do not require specific cofactors and have been shown to work in a wide range of species. These 3 pairs are the best characterized members from the family which have an identified excisionase.
From Mycobacterium phage Bxb1.
From Lactococcus lactis phage TP901-1.
Structure of the catalytic domain: [3BVP]
- [[phiRV1]] [int: o06604, RDF: o06606]
From Mycobacterium tuberculosis prophage-like element phiRv1.
 Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000. PMID 1123671.
 Control of phage Bxb1 excision by a novel recombination directionality factor., Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562.
 Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells., Russel JP et al., Biotechniques, 2006. PMID:16629393