JCA082006-Construction of lock3i, lock3j (J23071,J23072)

From OpenWetWare

Jump to: navigation, search

Construction of lock3 and lock3d variants with proper rbs spacing

PCR ca1000/ca1027R on pSB3C6-J01122 (179 bp, EcoRI/SpeI)
Sub into pSB3C6-J23018 (EcoRI/XbaI)
Product is pSB3C6-J23071, [Ptet][lock3i][RFP]

PCR ca1000/ca1027R on pJ23019-J23049 (319 bp, EcoRI/SpeI)
Sub into pSB3C6-J23018 (EcoRI/XbaI)
Product is pJ23019-J23072, [Ptet]-X-[lock3j][RFP]

Personal tools