- Added 9 ul GS500 size standard to each of four 1 mL tubes of formamide.
- Each well got 19 ul of formamide mixture plus 1 ul diluted PCR product
- Most of one tube is left over, kept in 4C for next round
- Spun down and denatured plates as usual
- Plate RUB101001A - runs 6256-6261 - panel 2 do-overs
- Run 6258 is columns 11-12 from plate SRUB100830A (transferred directly, no extra formamide added)
- Plate RUB101001B - runs 6262-6267 - panel 2 and panel 1 do-overs
- Syringe failure after run 6256. Lindsay replaced fill syringe, then Miki replaced reserve syringe next morning.
- Plates were stored in refrigerator overnight.