Jeff Tabor/MEGAWHOP

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Reaction setup

  • 120ng template plasmid
  • 500ng Megaprimer
  • Pfu Ultra II

Cycling conditions

95C, 2'
95C, 20"
55C, 20"
68C, 6-12'
25x

Transformation

  • DpnI, 1-2h
  • Qiaclean the reaction
  • Transform 0.5-2uL clean DNA into 50uL chemically competent E.coli
  • Plate all
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