Kubke Lab:/Notebook/Cranial nerve development/2010/12/16
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Meeting with Malisha regarding the planning of the work around the christmas holidays and the new building works schedule --MF Kubke 18:02, 15 December 2010 (EST)
MH004 Tried a few settings on the cryostat. Based on unstained check under the microscope, I seem to have gotten good quality by changing the knife angle to 0 - cut sections between 25-40 microns at 17 C. Tried colder but got a feeling they are more cracky, so stuck with 17C.--MF Kubke 23:20, 15 December 2010 (EST)
The afternoon was spent observing Fabiana's sectioning of MH004 alongside Malisha. Meanwhile I also performed a Nissl stain on slides 5 & 4 from embryo MH003 (incorrectly labeled as MH-004) which had been mounted on Tuesday afternoon by Malisha. Slides 5 and 4 are currently stored in the slide folder labeled RC003.
I have moved these slides into MH003 folder. Thanks for the note reuben--Malisha Hettiarachchi 20:58, 3 February 2011 (EST).