Kubke Lab:/Notebook/Cranial nerve development/2010/12/20

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General Entries

  • Afternoon end of year lab BBQ at Peter Thorne's. Please check your email for details.

Personal Entries

Malisha

On leave --MF Kubke 17:46, 16 December 2010 (EST)

Reuben

10:30am Placed slides 1,2,3,4&5 (RC004) which were sectioned on Friday afternoon by me into a staining rack. The rack was then immersed in the staining solutions for the durations provided below:

(Note: this entry does not match the embryo page entry --MF Kubke 04:52, 3 February 2011 (EST))
(Note: Do you mean the 70%EtOH time? I will check my notebook on Monday and correct it.)
Solution Duration (mins)
70% EtOH 5
95% EtOH 5
100% EtOH 10
Xylene 30
100% EtOH 10
95% EtOH 10
70% EtOH 5
Tap Water 1
Cresyl Violet 15
Tap Water 1
70% EtOH 1
95% EtOH 1
100% EtOH 2
Xylene 20
Coverslipped Infinite
(Note: The standard is to do two passes through 100% alcohol and 3 passes through xylene --MF Kubke 05:11, 20 January 2011 (EST))
(Note: Please transfer this information ot the embryo page)

Did not complete work in the afternoon due to Christmas arrangements.

Fabiana

  • Dealt with lab business in the morning and then went to Peter's BBQ.



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