Kubke Lab:/Notebook/Cranial nerve development/2010/12/21
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On leave --MF Kubke 17:45, 16 December 2010 (EST)
Following a meeting with Fabiana regarding poor record keeping the entries for the past week were updated from my lab notebook (paper version). I collected all my slides containing the embryonic sections that I cut (excluding embryo RC003 which was staged and cryoprotected by me but cut by Malisha and Fabiana) and put them into labeled slide folders (two folders one labeled RC-001 and the other RC-002&RC-004).
1:00pm Began working on my personal objectives shared document. I am adding a table of all my benchmarks and objectives such that the progress made for each point can be noted, seen and checked off by myself and Fabiana. I am currently completing the table now filling in the progress for each point.--Reuben Cutfield 19:30, 20 December 2010 (EST)
2:30pm I have completed filling out my objectives table for the summer. I wrote comments on my progress for each objective mentioned.I will now be constructing a section in my shared document which will allow me to develop a research question for my project with my progress being observable. --Reuben Cutfield 20:33, 20 December 2010 (EST)
2:45pm Read: Developmental organization of the glossopharyngeal nucleus in the embryonic chick brainstem slice as revealed by optical sectioning recording, by (Sato et al 2002). Added a proposed question to the shared personal objectives document.
3:30pm Read: MOLECULAR PROFILING INDICATES AVIAN BRANCHIOMOTOR NUCLEI INVADE THE HINDBRAIN ALAR PLATE by Ju, Aroca, Luo, Puelles and Redies, 2004. Was helpful in some respects, made a few notes from it.
5:00pm Looked at slides 1-5 (RC004) with Fabiana. The sections on slide 5 were the best and were good enough quality for analysis. She has instructed me to go ahead and cut a few whole embryos using the same settings as in slide 5 which were:
NB: RC004 was a stage ST25 embryo.