Kubke Lab:/Notebook/Cranial nerve development/2011/02/04

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Malisha

Plan for today:

  • Complete entering embryo records including some results, type of stain and staining procedure--Malisha Hettiarachchi 21:53, 3 February 2011 (EST).

MH006- Must go back and stain these sections.
MH003

  • Complete the required headings in the neuroanatomy document.
  • Follow up embryo atlas

- Nice animation showing 3D embryo development [1]

-Trying to figure out a way to collate the information on google docs so both me and Reuben are able to contribute to the document more collaboratively --Malisha Hettiarachchi 19:55, 3 February 2011 (EST).

Histology

  • A nice video showing coronal sections of mouse brain Nissl stained [2].
  • Nissl stain of mice cerebellum [3]
  • Nissl stain of telencephalon [4] description found here
  • "Mammalia - Felis - Cat spinal cord smear Nissl " [5]

Using the following resource previously mentioned here a set of criteria will be created to assess the quality of the [stained] sections:

  1. Do the cell bodies appear violet to dark blue?
  2. Under higher magnification are subcellular organelles visible?
  3. Is there evidence of cell loss?


Questions to answer:

  • What would the neurons look like in the Nissl stained sections?

The neurons should be stained dark violet against a lighter background colour. The following image illustrates this :[6]

Reuben

  • 10:30am-12:30pm Cresyl violet stain of MH007. The Slides are drying on the benchtop.
  • 1:45pm Working online with Malisha.--Reuben Cutfield 19:53, 3 February 2011 (EST)

Fabiana

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