Kubke Lab:/Notebook/Cranial nerve development/2014/12/12

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Helen

Today I attempted to whole mount my hindbrain of the stage 17 embryo I dyed yesterday but I got to the last step of transferring the hind brain to the slide and it tore apart. I am not used to working with such a tiny hindbrain and it ended up becoming so clear through the glycerol washes that I thought on numerous occasions that I had lost the brain. This just proves that I need to start practicing more on these younger ages and practice a method of being able to easily see the cleared hindbrains eg working on a black surface. Whilst waiting for the glycerol to do its thing I read a really interesting journal article on the interactions between efferents and afferents of cranial nerves in zebra fish which proposes that the 4 nerves it studied, all had different methods of establishment, for example they said that in the trigeminal nerve it appeared that the afferents were required to break through into the hind brain and make way for the efferents to leave but in the 7th 9th and 10th nerves it appeared that the efferents left before the afferents got in and so it might be the other way around. I thought at first this basically answered my question so do I need to change my experiment, but Fabiana assured me that the experiment they did is different enough that we would still be answering different questions and even if not, I would still need to replicate it because they could be wrong (or chickens and zebra fish could just have very different methods of development) my gut feeling (and this is also based a bit on what I have seen so far) is that it is true about the trigeminal afferents developing earlier than the VII/VIII (they didn't talk about the VIIIth nerve in this particular study but I will be) and so it might very well be that the efferents guide the way for the VII/VIIi and the afferents for the V . Now that I think it about it that adds a layer of complexity that is interesting so even though when I first read the article I felt a little disheartened - like oh no they already know the answer, now that I have reflected on it I realise that is actually an assistance and I can use it as a reference for my experiments.

Fabiana

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