Kubke Lab:Research/CND/Records2010-2011Summer/MH001

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Cranial Nerve Development Experiment

Contents

Embryo details


Species: Gallus gallus domesticus
Embryo Name: MH001
Embryo stage:28
Staging description:

  • The limb-ends are a "pointer contour" or triangular as opposed to curved.
  • Beak or nose present therefore it cannot be a stage 27 when the beak is barely visible.
  • Forelimb and hindlimb has not reached the level of development in a stage 29. Justified by comparing the photograph as well as the description of the stage 29 embryo."Limbs: Wing bent in elbow. Second digit distinctly longer than the others."
   *Hamburger, V and Hamilton, HL (1951). "A series of normal stages in the development of the chick embryo". J. Morph. 88 (1): 49–92. doi:10.1002/jmor.1050880104.
  • Eye does not protrude out. (Unsure about this comment).


Fixation: PFA
Cryoprotection: Yes cryoprotected by leaving the embryo in 0.1M PB 30% Sucrose solution overnight.
Material label and storage: Labeled as MH001 and stored in a sucrose filled vial.

Experiment details


Objective:

  • Practiced cutting techniques which are described in detail here.
(Note: Please add the information here. Also, what do you mean by 'colder' colder than what? Please be more specific--MF Kubke 04:21, 3 February 2011 (EST))


Procedure:
Comments:

Results

From this exercise I learnt that the a colder environmental setting facilitates less curling of OCT therefore making it easier to mount sections onto the slide. Cutting slowly also improves sectioning.

Images

Summary

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