Kubke Lab:Research/CND/Records2010-2011Summer/RC011
Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC011
Embryo stage: ST26
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. (Notebook entry 10th January 2011). Staging has not been confirmed.
- Hindbrain groove present (>ST24).
- Toes demarcated (>ST25).
- Beak not present (<ST27)
Fixation:PFA
Cryoprotection: None
Material label and storage: Stored in vial filled with PFA labeled RC-011 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective:To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST26.
Images
Summary
This embryo may be used in further experiments as approximately a ST26 embryo however confirmation is needed.