Kubke Lab:Research/CND/Records2010-2011Summer/MH005

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Cranial Nerve Development Experiment

Contents

Embryo details


Species: Gallus gallus domesticus
Embryo Name: MH005
Embryo stage: ST28 - ST29
Staging description:
Fixation:
Cryoprotection: No
Material label and storage:

Experiment details


Objective: To determine what are the best parameters to cut a stage 28 embryo.
Procedure:

Cryostat Sectioning Cryostat settings (for a more detailed protocol visit Kubke_Lab:Cryomicrotomy)

Cryostat Histology
Knife
Day Cut14/1/2011
Knife Angle0.3 and 0
Chamber Temp-17C
Object Temp-17C
Glass SlidesSuperfrost plus slides
Plane of sectionCoronal
Number of slides12
ObservationsI found that when i waited for a minimum of 1-2 minutes little or no curling was present. When cutting quite fast the section would curl immediately.

(Include in your observations, eg ,were the sections serial, was any section lost, was quality assessed, etc)



Cresyl Violet staining
For more informattion see Kubke_Lab:Nissl_Stain_Protocol

Date
Defatting and rehydration step
SolutionTimeComments
Water
75% alcohol
95% alcohol
100% alcohol 1
100% alcohol 2
Xylene 1
Xylene 2
Xylene 3
Xylene 2
Xylene 1
100% alcohol 2
100% alcohol 1
95% alcohol
75% alcohol
Water
Staining and differentiation step
SolutionTimeComments
Water
Cresyl Violet
50% alcohol
70% alcohol acetic acid
95% alcohol
100% alcohol 1
100% alcohol 2
Xylene 1
Xylene 2
Xylene 3
Coverslip



Comments:

Results

see the following entry

Images

Summary

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