|Cranial Nerve Development||Experiment|
Staging description: Originally staged by Reuben as a ST20, but Fabiana restaged on 25/1as a ST19 and Fabiana according to the Hamburger and Hamilton (1951) staging system. The description below corresponds to Reuben's staging.
- Wing and leg buds barely formed.
- The leg is slightly longer than the wing.
- Allantois has
vesicularizedand is roughly the same size as the midbrain (indicative of ST20).
- (Note: what do you mean by vesicularised? --MF Kubke 00:39, 27 January 2011 (EST))
- (Note: This is how H&H worded it. I thought they were describing the spherical/vesicle-like form that it takes on at this stage and since the embryo's allantois looked the same as their images I chose to use the same term.)
(see Notebook entry 10th January 2011)
Cryoprotection: Was stored in PFA, staged in .9% saline and then replenished with PFA.
Material label and storage:Stored in vial filled with PFA labeled RC005 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Objective:To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Cryostat Sectioning Cryostat settings (for a more detailed protocol visit Kubke_Lab:Cryomicrotomy)
|Glass Slides||Gelatin Subbed|
|Plane of section||Saggital|
|Number of slides||9|
|Observations||The sections "jumped" onto the slide and the whole embryo was|
(Include in your observations, eg ,were the sections serial, was any section lost, was quality assessed, etc)
Embryo is approximately ST20.
This embryo may be used in further experiments as approximately a ST20 however confirmation is needed.