Kubke Lab:Research/CND/Records2010-2011Summer/RC008

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Cranial Nerve Development Experiment

Contents

Embryo details


Species: Gallus gallus domesticus
Embryo Name: RC008
Embryo stage: ST24
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. (Notebook entry 10th January 2011). Staging has not been confirmed.

  • Eye is pigmented (>ST21).
  • Leg bud and wing bud are roughly the same size.
  • Limb buds are distinctively longer than they are wider.
  • Dorsal contour of trunk is curved.
  • 2nd Visceral arch distinctly protrudes across the ventral surface of embryo.


Fixation: PFA
Cryoprotection: None.
Material label and storage: Stored in vial filled with PFA labeled RC-008 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.

Experiment details


Objective: To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:

Results

Embryo is approximately ST24

Images

Summary

This embryo may be used in further experiments as approximately a ST24 embryo however confirmation is needed.

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