Kubke Lab:Research/CND/Records2010-2011Summer/RC013

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Cranial Nerve Development Experiment

Contents

Embryo details


Species: Gallus gallus domesticus
Embryo Name: RC013
Embryo stage: ST30
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. (Notebook entry 10th January 2011). Staging has not been confirmed.

  • Shape of beak matches ST30.
  • There is a distinct dip in the hindbrain's curve which is present on ST29-ST30 embryos.
  • The forebrain is split along the midline running laong the rostro-caudal axis.
  • There are no dots in the eye only faint pigmentation around the perimeter (<ST31).
  • Morphology of the toes matches 30 more closely than 29.


Fixation: PFA
Cryoprotection: None.
Material label and storage: Stored in vial filled with PFA labeled RC-013 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.

Experiment details


Objective:To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:

Results

Embryo is approximately ST30.

Images

Summary

This embryo may be used in further experiments as approximately a ST30 embryo however confirmation is needed.

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