Lan:Notebook/sdAB/2010/01/12

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Fractional Factorial design for TG1

  • Fractional Factorial design for TG1 using a 27-3 design with a resolution of IV
  • Factors are:
  1. Optical density at inoculation
  2. Flask volume (aeration rate)
  3. Inoculum resuspended in fresh medium prior to inoculation
  4. MgSO4 concentration
  5. Iron concentration
  6. Glucose concentration
  7. Trace metal solution prepared all at once or separate
' X1 X2 X3 X4 X5 X6 X7
Flask volumeResuspend inoMgSO4Fe GlucoseMetal solution mixedino volume
Low5Not resuspended11010Separate0.1
High25Resuspended550025mixed0.3
middle point10Not resuspended2.510017.5mixed0.2
  • Matrix
Trial # X1 X2 X3 X4 X5 X6 X7
10000000
21000101
30100110
41100011
50010111
61010010
70110001
81110100
90001011
101001110
110101101
121101000
130011100
141011001
150111010
161111111
' X1 X2 X3 X4 X5 X6 X7
15Not resuspended11010Separate0.1
225Not resuspended11025Separate0.3
35Resuspended11025mixed0.1
425Resuspended11010mixed0.3
55Not resuspended51025mixed0.3
625Not resuspended51010mixed0.1
75Resuspended51010Separate0.3
825Resuspended51025Separate0.1
95Not resuspended150010mixed0.3
1025Not resuspended150025mixed0.1
115Resuspended150025Separate0.3
1225Resuspended150010Separate0.1
135Not resuspended550025Separate0.1
1425Not resuspended550010Separate0.3
155Resuspended550010mixed0.1
1625Resuspended550025mixed0.3


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