Lidstrom:Formate Assay via Formate Dehdyrogenase

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Overview

  • Formate concentrations can be measured using formate dehydrogenase.
  • As formate is converted to CO2, NAD+ is reduced to NADH and A340 increases.
    • Janet has seen excellent linearity between Vmax and mM formate down to about 0.1mM formate.

Steps =

  1. Prepare buffer
    1. make extra buffer to freeze so you don't have to re-make it in the future.
  2. Make formate standard(s)
  3. Dissolve NAD+
  4. Dissolve FDH
  5. Test linearity
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