Light my XYZ: Material from Ed
- isolation of PR called 31A08 from BAC. This paper includes abs spectra from membrane preps and pH measurements of media Image:Bactrhod Science00.pdf Also link for 31A08 sequence at NCBI accession number AF279106 []
- characterization of two other PRs called HOT 75m and HOT 0m that were gifts from Chon in Ed's lab on 080805 Image:Proteorhodops Nature01.pdf NCBI accession number AF349981 for HOT 75m4 [] NCBI accession number AF349978 for HOT 0m []
- point mutation (Q105?) to change pH gradient created in response to light Image:Spectraltuning EMBO03.pdf
- example from Archea in which pH gradient created by PR is directly hooked into chemotaxis by making chimera of PR:Che. Not at all clear that this is do-able in E. coli or some other tamed bact. Image:PRCheArch biochem03.pdf
- protocol for membrane preps Image:PRmembranepreps FEBS97.jpg
- protocol for abs spect in presence of trans-retinal Image:RetinalAbsSpect biochem99.pdf
Currently have 31A08, HOT 75m and HOT 0m from Chon in Ed's lab as well as protease deficient strain UT5600 [] to express them in.
31A08 CDS complement(50866..51615)
/note="light-driven proton pump; has the properties of a light-driven proton pump when expressed with retinal in Escherichia coli" /codon_start=1 /evidence=experimental /transl_table=11 /product="proteorhodopsin" /protein_id="AAG10475.1" /db_xref="GI:9971913" /translation="MKLLLILGSVIALPTFAAGGGDLDASDYTGVSFWLVTAALLAST VFFFVERDRVSAKWKTSLTVSGLVTGIAFWHYMYMRGVWIETGDSPTVFRYIDWLLTV PLLICEFYLILAAATNVAGSLFKKLLVGSLVMLVFGYMGEAGIMAAWPAFIIGCLAWV YMIYELWAGEGKSACNTASPAVQSAYNTMMYIIIFGWAIYPVGYFTGYLMGDGGSALN LNLIYNLADFVNKILFGLIIWNVAVKESSNA"
HOT Om CDS <1..>753
/codon_start=1 /transl_table=11 /product="proteorhodopsin" /protein_id="AAK30176.1" /db_xref="GI:13560723" /translation="TMGKLLLILGSVIALPTFAAGGGDLDASDYTGVSFWLVTAALLA STVFFFVERDRVSAKWKTSLTVSGLVTGIAFWHYMYMRGVWIETGDSPTVFRYIDWLL TVPLLICEFYLILAAATNVAAGLFKKLLVGSLVMLVFGYMGEAGIMNAWGAFVIGCLA WVYMIYELWAGEGKAACNTASPAVQSAYNTMMYIIIFGWAIYPVGYFTGYLMGDGGSA LNLNLIYNLADFVNKILFGLIIWNVAVKESSNA"
HOT 75m4 CDS <1..>756
/codon_start=1 /transl_table=11 /product="proteorhodopsin" /protein_id="AAK30179.1" /db_xref="GI:13560729" /translation="TMGKLLLILGSAIALPSFAAAGGDLDISDTVGVSFWLVTAGMLA ATVFFFVERDQVSAKWKTSLTVSGLITGIAFWHYLYMRGVWIDTGDTPTVFRYIDWLL TVPLQVVEFYLILAACTSVAASLFKKLLAGSLVMLGAGFAGEAGLAPVLPAFIIGMAG WLYMIYELYMGEGKAAVSTASPAVNSAYNAMMMIIVVGWAIYPAGYAAGYLMGGEGVY ASNLNLIYNLADFVNKILFGLIIWNVAVKESSNA"