Lissa1:PCR

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  1. 5 ul Thermopol Buffer
  2. .5 uL dNTPs
  3. .5 uL VENT (ADD LAST- keep on cold block)
  4. 2 uL primer1 (at 25 uM)
  5. 2 uL primer2 (same [])
  6. 1 uL template (50-100 ng)
  7. 39 uL water
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