Lissa1:Pouring Plates
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- Get 200 mL of 4% agar.
- Microwave agar.
- From kitchen, get 2x SD 200 mL (synthetic dropout – nitrogen/vitamins/minerals).
- Add liquid ingredients in SD flask.
- Add 40 mL 10x CSM (minus stuff) – you are diluting it to 1x. These are the amino acids.
- Add dextrose. It’s at 40%, you need to add 20 mL to make a 2% solution in 400 mL.
- Pour stuff from SD flask in to agar jar, then pour plates.