Livesey:SSPE
From OpenWetWare
Jump to navigationJump to search
20X SSPE BUFFER
3 Liters
525.9g NaCl sodium chloride
82.8g NaH2PO4 sodium phosphate monobasic
28.2g EDTA powder FW=372
bring up to 2400mls with H2O
add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
autoclave for 20 min
2 Liters
350.6g NaCl sodium chloride
55.2g NaH2PO4 sodium phosphate monobasic
18.8g EDTA powder FW=372
bring up to 1600mls with H2O
add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
autoclave for 20 min
1 Liters
175.3g NaCl sodium chloride
27.6g NaH2PO4 sodium phosphate monobasic
9.4g EDTA powder FW=372
bring up to 800mls with H2O
add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
autoclave for 20 min
