Magni:Protocols/LB

From OpenWetWare

Jump to: navigation, search

Home        Contact        Lab Members        Publications        Research        Internal        Protocols        Data       


LB medium (liquid)

  • For every 1 L of medium, add to a pyrex bottle or flask:
    • 10 g NaCl
    • 10 g bacto-tryptone
    • 5 g yeast extract
  • Add 1 L of deionized water (blue tap of the Millipore machine).
  • Autoclave (if using a bottle, do not close the cap completely).
  • Let it cool to about 40degC and, when required, add antibiotic near the flame.
  • Store in the fridge and always check for contaminations before using.

LB medium (solid)

  • For every 1 L of medium, add to a pyrex bottle or flask:
    • 10 g NaCl
    • 10 g bacto-tryptone
    • 5 g yeast extract
    • 15 g of agar
  • Add 1 L of deionized water (blue tap of the Millipore machine).
  • Autoclave (if using a bottle, do not close the cap completely).
  • Let it cool to about 40degC and, when required, add antibiotic near the flame.
  • Pour approximately 20 ml of medium in Petri dishes and let them polymerize at room temperature.
  • Cover with foil or parafilm, store in the fridge and always check for contaminations before using.
Personal tools