Protocol for Nucleic Acid Precipitation from Diluted Solutions
- Add 1/10 volume of 3M sodium acetate (or 2M sodium chloride, or 5M ammonium acetate) to DNA solution.
- Add Glycogen to a final 0.05-1µg/µl concentration. Use up to 1µl of Glycogen per 20µl of the solution.
- Add 1 volume of isopropanol (or 2.5 volumes of ethanol) to the solution. Mix gently but thoroughly. Ethanol is recommended for precipitation of smaller than 200 b/bp RNA/DNA fragments.
- Incubate the mixture at -20°C for up to 60min, or at -70°C for 30min.
- Centrifuge the mixture for 10-15min at 10,000rpm. Discard the supernatant.
- Rinse the pellet with cold 70% ethanol. Air-dry the pellet.
- Dissolve DNA in Water, nuclease-free <http://www.fermentas.com/catalog/reagents/water.htm> (#R0581) or TE buffer.
- Dissolve RNA in DEPC-treated Water <http://www.fermentas.com/catalog/reagents/depc.htm> (#R0601).
Using only sodium acetate and ethanol works well - CJZ 6/24/10